Column Method

• Simply P Total RNA Extraction kit MORE
  Product Details：

  Kit Components

  Cat#	BSC52S1	BSC52M1
  Components	50Tests	100Tests
  Solution R1	10ml	20ml
  Solution R2	30ml	60ml
  Wash Buffer	25ml (add 75ml ethanol before use)	25ml×2 (add 75 ml ethanol before use)
  Elution Buffer	10ml	20ml
  Spin columns	50	100
  Handbook	1copy	1copy

  Introduction

      The kit is a ready-to-use reagent for the isolation of total RNA from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. Add Solution R2 to the processed sample and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis ,construction cDNA library etc.

  Product Features：

  Storage and transportation

  •  The kit has demonstrated stability of 24 months when stored at room temperature.
  •  The kit can be transported at room temperature.

  Technical Information

  Method	Time	Max volume	Yield
  Spin column	7~15min	750µl	≥90％

  Apparatus and materials to be prepared by the user

  *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000rpm                          * Absolute ethanol

  *  Vortex mixer

  Important note

  Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

  Experimental data:

  Analysis RNA

  Absorbance analysis of yield and purity

  lPrepare RNA, dilute by 25mM Tris-HCl（pH7.5）, RNase-free water or TE buffer in a right factor；

  lZero the spectrophotometer at 260 and 280nm with 25mm Tris-HCl, RNase-free water or TE buffer；

  lMeasure the OD using 100μl the RNA diluent solution, calculate the concentration of RNA as follows:

  Final concentration = (Spec reading A260) × (Dilution factor)  × (Conversion factor A260)

  ð The conversion factor for RNA is 0.040μg/μl per OD260 unit

  ð Notice: the range of Spec reading A260： 0.1≤OD260≤1.0

  Calculate the purity of RNA as follows: Ratio= (Spec.readingA260)/ (Spec.readingA280)

  ð Ration of 1.8~2.0 are considered ideal purity.

  （The low pH will alter the OD measurements between 260 and 280nm, indicating a low purity.）

  Picture 1：Animal

  

  Manual download