introduction
real-time pcr detection system is up-to-date product based on line-gene. by using the advanced optical fiber conducting technique, the unit has strong sensitivity and reliability when detects the fluorescence. at the same time, it inherits all merits of line-gene so to realize speedy and accurate pcr detection. what's more, the powerful software provides multiple channels fluorescence detection with four kinds of dyes at the same time. and the testing result of samples can be analyzed and compared by groups so that many functions, such as absolute quantitative, comparative quantitative, snps and melting curve analysis, can be accomplished.
multiple applications
taqman probe; sybr green i; molecular beacons, fam, hex, tamra, texasred, rox and so on.
detection format |
detection channel |
reporter dye |
simple probe |
530 nm |
fluorescein |
taqman format |
530 nm |
fam |
sybr green i |
530 nm |
sybr green i |
hybridization probe |
640 nm
710 nm |
lc 640
lc 705 |
|
features
·multicolor fluorescence detecting
· coaxial optical fiber conducting
· wide range of detection linearity
· long life blue led light source
· extremely sensitive pmt detection module
· sandwich thermo-electronic (te) module
· powerful software with multiple functions
working principle:
heating & cooling unit
① special designed te module for pcr application has a long life as many tines-times as that of normal products.
② advanced pid controlling technique ensures the accuracy of temperature control.
③ unique sandwich heating &cooling structure nakes temperature change much quicker and more uniformer.
④ using the hot-lid realizes oil free operation.
optical unit
① special bottom detecting method can shorten light length and strengthen sensitivity.
② adopting coaxial optical fiber conducting technique can reduce the interference between excitation light and acceptance light and also increases the ratio between signals and noises.
③ using multiple groups of filters to detect many kinds of fluorescence simultaneously.
④ high-powered pmt has a sensitive detecting ability.
⑤ long-lived led excitation light source does not need maintenance or preheating.
multicolor fluorescence detection
with multiple groups of filters, the unit can detect many kinds of fluorescence simultaneously. the operator can setup four(4) channels at the same time by changing specific filter groups to meet different demands of kits and dyes such as molecular beacons, taqman, fam, sybr green, hex, tamra, rox and so on.
snps detection
system is a multicolor fluorescence detection system and can carry out the detection analysis of snps.
relative quantification
when doing the relative quantification, the instrument doesn't make the standard curve, however, it offers the relative expression level of samples by comparing to inner standards. the relative quantitative detection procedure and result analysis are all finished automatically by software of .
samples' grouping
through this function, user can divide sample wells into many groups at will in order to do different experiments so that you can analysze respective positive standards independently in every group and can compare every sample data among one group. |