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line-gene ⅱreal-time pcr detection system
 
 
 
Instruments
Consumables

 specifications


introduction
real-time pcr detection system is up-to-date product based on line-gene. by using the advanced optical fiber conducting technique, the unit has strong sensitivity and reliability when detects the fluorescence. at the same time, it inherits all merits of line-gene so to realize speedy and accurate pcr detection. what's more, the powerful software provides multiple channels fluorescence detection with four kinds of dyes at the same time. and the testing result of samples can be analyzed and compared by groups so that many functions, such as absolute quantitative, comparative quantitative, snps and melting curve analysis, can be accomplished.

multiple applications

taqman probe; sybr green i; molecular beacons, fam, hex, tamra, texasred, rox and so on.

detection format

detection channel

reporter dye

simple probe

530 nm

fluorescein

taqman format

530 nm

fam

sybr green i

530 nm

sybr green i

hybridization probe

640 nm

710 nm

lc 640
lc 705

features
·multicolor fluorescence detecting
· coaxial optical fiber conducting
· wide range of detection linearity
· long life blue led light source
· extremely sensitive pmt detection module
· sandwich thermo-electronic (te) module
· powerful software with multiple functions

working principle:

heating & cooling unit
① special designed te module for pcr application has a long life as many tines-times as that of normal products.
② advanced pid controlling technique ensures the accuracy of temperature control.
③ unique sandwich heating &cooling structure nakes temperature change much quicker and more uniformer.
④ using the hot-lid realizes oil free operation.

optical unit
① special bottom detecting method can shorten light length and strengthen sensitivity.
② adopting coaxial optical fiber conducting technique can reduce the interference between excitation light and acceptance light and also increases the ratio between signals and noises.
③ using multiple groups of filters to detect many kinds of fluorescence simultaneously.
④ high-powered pmt has a sensitive detecting ability.
⑤ long-lived led excitation light source does not need maintenance or preheating.

multicolor fluorescence detection
with multiple groups of filters, the unit can detect many kinds of fluorescence simultaneously. the operator can setup four(4) channels at the same time by changing specific filter groups to meet different demands of kits and dyes such as molecular beacons, taqman, fam, sybr green, hex, tamra, rox and so on.

snps detection
system is a multicolor fluorescence detection system and can carry out the detection analysis of snps.

relative quantification
when doing the relative quantification, the instrument doesn't make the standard curve, however, it offers the relative expression level of samples by comparing to inner standards. the relative quantitative detection procedure and result analysis are all finished automatically by software of .

samples' grouping
through this function, user can divide sample wells into many groups at will in order to do different experiments so that you can analysze respective positive standards independently in every group and can compare every sample data among one group.

 

 


the same function of data inquiry system with

·specification

model

fqd-66a

sample capability

66×0.2ml

dynamic range

copies

sample volume range

10-100μl

temp. range

30℃~99℃

heating rate(max)
≥ 4.0°c /sec

cooling rate

≥ 4.0°c /sec

block temp. uniformity

≤±0.5℃

block temp. accuracy

≤± 0.5℃

temp. range of hot lid
105℃

detecting fluorescence

fam, sybr greenⅰ,hex,vic(tamra,tex red,rox can be customized)

operation system

windows 2000/xp

power supply

ac220v 50hz 800w

dimension (mm)

550×520×270(l×w×h)

net weight

27kg(without computer)



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