line-gene. k is the newest system in bioer's range of pcr detection systems. it features more advanced implementations of peltier and fiber optic technology and a new direct current power system.
the new instruments share the peltier effect and fiber optic design features that provide the exceptional accuracy and stability of the line-gene instruments.the new units offer improved uniformity,stability and accuracy by utilizing a constant-current power supply and an advanced multi-point temperature control module.the line-gene.k has improved temperature control and more uniform heating throughout the sample block to improve the accuracy of pcr detection.
the line-gene.k series is available in a range of automated and manual models with the ability to handle one, two, three or four combinations of excitation and emission wavelengths.
15 items(just for you!) ●volume adaptation—software automatically adjusts heat exchange to differences in sample volumes.
●new multipoint temperature monitoring allows more accurate and uniform temperature control throughout the heating block.
●gradient function—creates a temperature gradient across the sample block. maximum 24℃ difference between row 1 and row 24.
●sample preservation—after completion of experiment, the system can automatically refrigerate samples until they are moved to storage.
●use of direct current power supply improves thermal efficiency and reduces power consumption by 30%.
●improved optics—greater efficiency in fiber optic system provides reduced signal to noise ratio.
●automatic hot lid—provides constant pressure and software-managed temperature control.
●configuration upgradable—manual versions of the instrument can be upgraded to automatic. systems can be upgraded from 1 wavelength to 4 wavelengths.
●numerous data ports—choose from serial(rs 232),usb and ethernet connections for pc data interface.
●multiple tube styles—the sample block will accept several styles of 0.2ml tubes including 8-tube strips.
●data protection—system preserves experimental data if there is a power interruption during operation.
●automatic amplitude adjustment—the instrument detects the fluorescence strength in the samples and automatically adjusts to the correct system sensitivity.
●new software—a new generation of line-gene software provides even greater convenience and more powerful functions.
●upload software instructions—define experiment instructions with easy-to-use pc- based software,then upload to instrument.
●overheating protection—if the operating environment exceeds the temperature range for operation within specification, the instrument will shut down.at 35℃the instrument will sound a warning;it will turn off at 40℃.
interface introduction:
software:
snps detection
line-gene system is a multicolor fluorescence detection system and can carry out the detection analysis of snps.
two kinds of data analysis methods:
2nd derivative maximum:
this method automatically calculates the maximum second derivative value of every fluorescent curve and defines this value as the ct value.
fit points:
the user sets baseline and a threshold values then draws a threshold line.based on user instructions,the software will select a number of sample points on the fluorescence curve that are above baseline and in the logarithmic phase into a line.the intersection of this line with the threshold line is defined as the ct value.
specifications:
model
fqd-48a(a4) (a4: means automatic
lid and 4 channels)
fqd-48a(m2) (m2: means manual
lid and 2 channels)
sample capacity
48×0.2ml
48×0.2ml
reaction system
10~100μl
10~100μl
dynamics range
10~1010copies
10~1010copies
max.channel number of
fluorescence detection
4 channels
2 channels
excitation light wavel
ength(450-590mm)
standard positioning：
f1:470nm f2:523nm
f3:543n f4:571nm
f1:470nm f2:523nm
fluorescence detecting
wavelength(500-630nm)
standard positioning：
f1:525nm f2:564nm
f3:584n f4:612nm
