magabio general genomic dna dna purification kit provides a very simple, fast and cost-effective technique to isolate high quality dna. using one simple protocol, high yield of purified dna can be isolated from various sources including whole blood, buffy coat, leukocytes and cultured cells. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure dna can be applied extensively in pcr, sequencing, southern hybridization, mutant analysis, snp and the others.
according to the special interaction, use magabio nucleic acid separation system with a general protocol---sample processing, magabio adsorption, washing and elution, and can go high-throughput.
attribute:
| item |
description |
specification |
50t and 100t |
max. volume of sample |
2ml blood, 50mg animal tissue,1¡Á108 cultured cell |
mix. volume of sample |
10-20 ¦Ìg blood, 2mg animal tissue,5¡Á108 cultured cell |
dna performance |
2¦Ìg - 6¦Ìg / 200¦Ìl blood, 2-15¦Ìg / 20mg animal tissue, 2-8¦Ìg / 5¡Á108 cultured cell |
dna purity |
1.7~1.9 (a260/a280) |
operation time |
less than 35min ( 16 samples with machine model) |
less than 45 min (2-12 samples with manual model) |
accessory |
magnetic rack |
machine |
npa-32 nucleic acid purification machine |
features
first elution can acquire >85%
no organic and high salt solvent
suitable for automatic platform
simple, effective and safe process, no centrifugation
especially used for mini-quantity sample, for example with the volume less than 5mg tissue or 50ul blood
experimental data
human blood genomic dna purification
electrophoresis results |
mouse tissue genomic dna purification
electrophoresis results |
m ¦Ë dna/hind ¢£ marker
1,2,3,4,5,6 100¦Ìl blood |
m ¦Ë dna/hind ¢£ marker
1,2,3,4,5,6 20mg mouse liver tissue |
order information
| cat# |
name |
specification |
remarks |
bsc07s2 |
magabio general genomic dna purification kit |
50t |
stored at room temperature
(protease k stored at 2~8 ¡æ) |
bsc07m2 |
magabio general genomic dna purification kit |
100t |
|
