the magabio total rna purification kit provides a very simple, fast and effective technique to isolate high quality rna. by one simple protocol, purified rna in high yield can be isolated from various sources including whole blood, leukocytes and tissue. magabio sample processing is based on proprietary magnetizable particles--magabio. the pure rna can be applied extensively in rt-pcr, northern hybridization, mutant analysis, rnai and the others.
attribute:
| item |
description |
specification |
50t and 100t |
the volume of sample |
10¦Ìl£5ml blood, 2mg-20mgtissue |
rna performance |
1-5¦Ìg/1ml whole blood 0.5¦Ìg/10 mg liver |
rna purity |
1.7~1.9 (a260/a280) |
fragment size |
2 kb(18s) and 5 kb (28s) |
operation time |
less than 40 min ( 16 samples with machine model) |
less than 55 min (2-12 samples with manual model) |
accessory |
magnetic rack |
machine |
npa-32 nucleic acid purification machine |
features:
suitable for most of sample, excellent adaptability
no organic solvent and other toxic reagent
suitable for automatic platform
simple, effective and safe process
especially used for mini-quantity sample, the volume less than 5 mg tissue or 50ul blood
procedure
lysis binding washing elution
experimental data:
tissue&blood rna purification results
tissue total rna purification |
blood purification results |
1,2,3 10mg liver tissue total rna
4,5,6 10mg muscle tissue total rna |
5 ml blood red cells lysis buffer
concentration for purification total rna |
liver tissue total rna purification rt-pcr
1, 2 10mg liver tissue 3, 4 5mg liver tissue 5, 6 3mg liver tissue
6 samples total rna purification, the sample volume were 10mg/5mg/3mg respectively, use quantitative pcr to gain the initial copy number
order information:
| cat# |
name |
specification |
remark |
bsc53s2 |
magabio total rna purification kit |
50t |
stored at room temperature
(protease k stored at 2~8 ¡æ) |
bsc53m2 |
magabio total rna purification kit |
100t |
