the kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. plasmid dna can be purified from 1~5ml of overnight cultures of e. coli. the dna isolated by this kit is ready for downstream applications such as restriction enzyme digestion, sequencing, pcr/real-time pcr and other downstream experiments.
attribute:
item
description
specification
50t and 100t
sample volume
from 15ml of overnight cultures of e.coli.
dna performance
up to 20 ¦Ìg
dna purity
1.7~1.9 (a260/a280)
operation time
< 25 min
procedure
experiment data
¢Ùsample:
pgem -7(+)/jm 109, 37¡æ for 16 times; 1~3# is the plasmid dna isolated from 3ml cultures.
¢Úcompared with q company (1 ml culture)
¢Û examples of downstream experimen
1. restriction enzyme digestion (pgem-7(+))
2. real-time pcr (the templet is pgem-7(+))
b: biospin q: the product from q company
order information
cat#
name
specification
remarks
bsc01s1
biospin plasmid dna extraction kit
50t
stored at room temperature
(rnase a buffer stored at -15¡æ)
