biospin gel extraction kit provides a simple, rapid and effective method to purify dna fragments from agarose gel in tae or tbe buffer. dna fragments ranged from 60bp to 23kb are purified from (up to 3% standard or high/low-melt) gel using spin column. purified dna can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on.
attribute:
item
description
specification
50t and 100t
sample volume
¡Ü400mg gel slice
dna fragment range
60bp~23kb
buffer kind
tae; tbe
incubate temperature
50¡æ
gel consistence
¡Ü3%
operation time
¡Ü15 min
procedure
gel slice containing dna fragment
extract
bind
wash
elute
purified dna
experimental data
1.sample: 1007bp pcr product 1~10# are products after extraction. marker: dl2000
contrast of the absorbance result before and after extraction: according to the contrast of gray scale, the yield is 95%
2.the following pictures show the extraction result of different dna fragments.
¡õbefore extraction ¡õafter extraction