biospin bacteria genomic dna extraction kit provides a very simple, fast and economic way to isolate pure high–molecular-weight genomic dna from all kinds of bacterial, including gram-negative bacterial and gram-positive bacterial. the simple purification procedure, based on the remarkable selectivity of biospin membrane, allows isolation of high yields of pure genomic dna less than 1 hour. it doesn’t require any expensive equipment, or toxic reagents. in general, the kit can get at most 30ug genomic dna from 5×109 bacterial cells. the pure dna can be applied extensively in pcr/real-time pcr, sequencing, southern blot, mutant analysis, snp and the others.

attribute:

item	description
specification	50t and 100t
dna performance	5~30 μg/ less than 5×109
max. sample volume	5×109 bacterial cell
sample source	cultured yeast, liquid fungus cultures, fruiting body, spore
dna purity	1.7~1.9 (a260/a280)
operation time	≤60 min

procedure
collected bacteria

equilibrating sample

lysis and pk action

impurity destroy

bind

wash

elute

purified genomic dna

experimental data:
sample: 1/2 colibacillus 3/4 gonococcus 5/6 bacillus subilis

b: biospin q: the product from q company
w: the product from w company t: the product from t company

colibacillus

gonococcus

bacillus subilis

staphylococcus aurus

order information

cat#	name	specification	remarks
bsc12s1	biospin bacteria genomic dna extraction kit	50t	stored at room temperature (proteasek stored at 2~8℃)
bsc12m1	biospin bacteria genomic dna extraction kit	100t