biospin polyacrylamide gel dna extraction kit provides a simple, rapid and effective method to purify dna fragments from polyacrylamide gel. the simple purification procedure is based on the remarkable selectivity of biospin membrane and won¡¯t affect the results when different staining methods are used. purified dna can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on.
attribute:
item
description
specification
50t and 100t
staining method
eb, silver, sybr
dna fragment range
60bp~10kb
dna purity
1.8~2.0 (a260/a280)
recommend gel consistence
6%~20%
procedure gel slice containing dna fragment
spall gel and incubate at 65¡æ
add binding buffer
bind
wash
elute
purified dna
experimental data
result of different dna fragments
m: dl2000 marker s: before extraction r: after extraction
comparison among different brands: b:biospin o: the product from o company t: the product from t company
compare biospin with o
be: biospin eb staining
ba: biospin silver staining (fix)
m: dl2000 marker
oe: o eb staining
oe: o silver staining (fix)
compare biospin with t
be: biospin eb staining
ba: biospin silver staining (fix)
m: dl2000 marker
te: t eb staining eb
te: t silver staining (fix)
