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Life Science Reagents
 
 
Nucleic Acid Purification Series
Spin Column Extraction Kits
  PCR Kits
General Kits

simply p total rna extraction kit is a ready-to-use reagent to isolate total rna from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. add solution r2 to the processed sample and transfer the mixture to spin column, and then total rna can be easily isolated through several washing and eluting steps. simply p total rna extraction kit provides a very simple, fast and economical technique to isolate high quality rna in high-throughput. the pure rna can be applied extensively in northern blot, blotting hybridization, poly(a)+ selection ¡¢in vitro translation ¡¢rnase protect assay, rt-pcr/real time rt-pcr analysis¡¢construction cdna library etc.

features:
fast: ready-to-use high quality rna within 9 minutes
simple: only 4 steps for extraction rna from samples
easy: ready-to-use reagents. no need of professional during extraction operation.
no phemol/chloroform extraction, no cscl gradients, no licl or ethanol precipitation.
suit for many kinds of samples
excellent recovery of rna of starting samples from very small to large amount.

method

spin column

time

7~15min

max volume

750¦Ìl

yield

¡Ý90£¥

sample amount

blood 0.1~10ml
animal/plant tissue 3~30mg
cultured cell ¡Ü1¡Á107cell
body fluid 1~10ml
bacteria 1ml

contrast

traditional reagents(trizol, biozol)

others company¡¯s production

simply p

acid lysis buffer

acid lysis buffer

neutral lysis buffer

stored at 4¡æ for 1 year

stored at 4¡æ for 1 year

stored at rt for 2 year

finish extraction within 30 minutes

finish extraction within 20 minutes

finish extraction within 9 minutes

mouse liver total rna extraction

order information

cat#

name

specification

remarks

bsc52s2

simply p total rna extraction kit

50t

centrifuge column

bsc52m2

simply p total rna extraction kit

100t

   


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