hepatitis c virus is considered to be the principal etiologic agent responsible for 90-95% of the cases of post-transfusion non-a and non-b hepatitis. hcv is a single-stranded, positive sense rna virus with a genome of approximately 10,000 nucleotides coding for 3,000 amino acids. as a blood-borne virus, hcv can be transmitted by blood and blood products. the global prevalence of hcv infection, as determined by immunoserology, ranges from 0.6% in canada to 1.5% in japan.
serological screening assays have greatly reduced, but not completely eliminated, the risk of transmitting viral infections by transfusion of blood products. recent studies indicate that nucleic acid-based amplification tests for hcv rna will allow detection of hcv infection earlier than the current antibody based tests. nucleic acid testing of whole blood donations has been in place in the united states since 1999 under investigational new drug application. nucleic acid-based tests can detect the units of virus donated by carriers who do not seroconvert or who lack antibodies to serological markers normally detected by immunological assays.
this fluorescence detection kit is adapted for many kinds of real time pcr detection instrument, specifically adapted for line-gene i&ii real-time pcr detection system. hcv rna is reverse transcribed and a specifoc fragment is amplified with specific primers in a one-step rt-pcr reaction. the products are detected by using a specific taqman-mgb probes. this taqman-mgb probes is labeled at the 5’-end with fam report dye, and the 3’-end by nfq (non fluorescent quencher)-mgb.

except for lysis reagent store at 4℃,the others store at -20℃. the kit can be stored for up to 12 months if all components are kept in the manner above.

applications
chart 1 real-time pcr curve

chart2 standard curve

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