numerous data on telomerase expression demonstrate the presence of telomerase activity in the vast majority of different types of cancer - as well as immortalized cells -but fail to detect telomerase in most normal tissues. using the highly sensitive pcr-based trap method, telomerase activity has been detected in most neoplastic lesions and appears to be necessary for the sustained proliferation of most advanced cancers. examples include breast cancer, neuroblastoma, and cervical cancers where a correlation between telomerase expression and the stage of the disease has been demonstrated. therefore the presence of telomerase enzyme activity in tumors might lead to a useful marker for diagnosis and prognosis of cancer in the future. determination of expression levels of telomerase-encoding mrna presents an alternative approach to detect telomerase in various sample materials.the human telomerase reverse transcriptase (htert) mrna one step rt-pcr quantification kit is intended as research tool facilitating the generation of data to proof these hypothesis¡¯s.
this quantification kit is adapted for many kinds of real time pcr detection instrument., specifically adapted for line-gene i&ii&k real-time pcr detection system.htert -encoding mrna is reverse transcribed and a fragment of the generated cdna is amplified with specific primers in a one-step rt-pcr reaction.the amplicon is detected by using a specific taqman-mgb probes.this taqman-mgb probes is labeled at the 5¡¯-end with fam report dye , and the 3¡¯-end by nfq(non fluorescent quencher)-mgb.
