bioeasy sybr green i real-time pcr kit can detect and quantify the target gene fast and properly. the kit offers 2¡Ásybr mix, including taq dna polymerase and ddh2o. during the operation, user can do real-time pcr reaction directly by adding primer, template and taq dna polymerase. sybr green i is one kind of fluorescence dye, which will excited fluorescence signal after specially inserted in dsdna molecule. in pcr reaction system, sybr green i dye will combine with dsdna, then excite. via testing strength of fluorescence, user can veracious calculate the quantity of dsdna. by the analysis of melt curve, user can judge whether there are existing aberrance or non-specific amplification. the max absorb wave length of sybr green i is 497nm, the max. emission wavelength is about 520nm.
components |
volume/ul |
quantity |
2¡Ásybr mix(with 4.0mm mg2+) |
1250 |
2 |
taq polymerase |
32 |
1 |
25mmmgcl2 |
1000 |
1 |
ddh2o |
1000 |
2 |
experimental data
¢Ù plasmid dna template pcr, copies area of sample 4-10 is in 10~107 copies/ml. length of pcr product is 177bp
¢Ú cdna(rna) pcr, concentration area of sample 4~9 is in 100ng~1pg. length of product is 555bp.
¢Ûhuman genomic dna pcr ,concentration area of sample 4~8 is in 1 ¦Ìg-100¦Ìg.length of pcr product is 243 bp
¢Üresults from abi 7900 real-time pcr system
order information
| cat# |
name |
specification |
remarks |
bsb03m1 |
bioeasy sybr green i real-time pcr kit |
100t |
stored at -15~ -25¡æ |
bsb03l1 |
bioeasy sybr green i real-time pcr kit |
200t |
