rt-pcr (reverse transcription-polymerase chain reaction) is a technique in which an rna strand is "reverses" transcribed into its dna complement, followed by amplification of the resulting dna using a polymerase chain reaction (pcr). rt-pcr can be used to examine gene expression level in cells and tissues, clone the specific gene of cdna sequences and test rna viruses.
biort two step rt-pcr kit has been designed for two-step rt-pcr of rna samples from various sources, such as total rna, messenger rna, viral rna and in vitro transcribed rna, all which can be used as a template for reverse transcription. the kit includes both random primers and oligo(dt18) primers. the pcr’er can choose either of them or choose a specific primer instead. our kit adopts high-quality amv reverse transcriptase produced in usa and high-fidelity taq mix dna polymerase. this complete and ready-to-use kit contains an enhanced viral reverse transcriptase (higher thermal stability up to 60℃ rt temperature), and providing highly sensitive synthesis of full-length cdna (up to 12kb) even from low-abundance targets. the buffer is specially formulated to provide efficient reverse transcription with no optimization required. the novel rnase h activity quencher binds to the cdna:rna hybrid–binding site of the reverse transcriptase preventing degradation of the rna during synthesis and enabling generation of full-length cdna. the biort two step rt-pcr kit also contains all of the components necesssary for subsequent pcr. the performance of the pcr step is based on the high fidelity taq mix dna polymerase which can get the dna up to 6kb. the reaction buffer is optimized for two kinds of reaction system (10ul for rt and 25ul for pcr, or 20ul for rt and 50ul for pcr).

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