unlike two steps rt-pcr methods, in which rt and pcr separated in two tubes, biort one step rt-pcr kit is designed for easy and sensitive one-step rt-pcr using any rna template. a unique enzyme combination and specially developed reaction buffer ensure efficient, highly specific reverse transcription and pcr in one tube, without the need for optimization. our kits adopt high-quality amv reverse transcriptase produced in usa and taq polymerase. we introduce an optimized buffer to ensure the maximize effectiveness of these two enzymes. cooperation of amv reverse transcriptase and highly purified recombinant taq polymerase can obtain 4kb extent of products biort one step rt-pcr kit adopts one tube system. because operator doesn’t need to open the lid during the reaction process, this user-friendly improved version avoids cross contamination and has the merits of high specificity and sensitivity. biort onestep rt-pcr kit is suitable for rt-pcr applications such as: gene-expression analysis，virus detection ，single-cell rt-pcr，etc. features
simplicity - fast and easy one-tube setup
minimal optimization - efficient reverse transcription and specific amplification save time and money
sensitivity - unique blend of two high-affinity reverse transcriptases and a hot-start pcr enzyme
robustness - combination of an optimized one-step rt-pcr buffer with a balanced mixture of enzymes
experimental data
rna template that is diluted by grads has been done rt-pcr by biort one step rt-pcr kit. (450bp)
1 μg mouse liver total rna has been done rt-pcr respectively by biort one step rt-pcr kit
1 μg mouse total rna is done rt-pcr by biort one step rt-pcr kit
