BioReady Taq is a highly pure, thermostable recombinant DNA polymerase encoded by a modified gene from Thermus aquaticus species and expressed in E.coli. Its recombinant nature ensures utmost purity, reproducibility and processivity. BioReady Taq processes an initial 5’→3’ exonuclease activity and lacks 3’ →5’exonuclease activity. The enzyme leaves a single 3’-nucleotide overhang that makes the product for cloning by TA vector system. BioReady Taq provides a strong thermostability that meets the requirements of specialized PCR applications.
This product includes all components except primers. Users only need to prepare primers and template to run PCR. The operation is rapid and simple.
Components
BSA09S2
BSA09M2
BioReady Taq.
200units
BioReady Taq.
500units
10×reaction buffer(with 15 mM MgCl2 )
500ul
10×reaction buffer(with 15 mM MgCl2 )
1000ul
2.5 mM dNTP Mixture
350ul
2.5 mM dNTP Mixture
800ul
25 mM MgCl2
500ul
25 mM MgCl2
500ul
6×loading dye
300ul
6×loading dye
500ul
ddH2O
1250ul
ddH2O
1250ul×2
Features
Good thermostable
High activity
The PCR product with this enzyme is available to run TA clone directly
The product is suitable for real-time PCR reaction owing to 5’-3’ exonuclease activity.
Application
PCR raction
TA clone
Sequence
Experimental Data
1. Length of PCR product and PCR efficiency 2. BioReady Taq in Real-Time PCR
Lane1:2000bp
Lane2:1000bp
Lane3:500bp
Lane4:100bp
M: DL 2000
