The BioReady Hot Start Taq is a developed method to minimize the side effects during PCR. The BioReady Hot Start Taq inhibits Taq polymerase activity during RCP reaction preparation. By limiting Taq polymerase activity at low temperature prior to PCR cycling, non-specific amplification and primer-dimers are reduced.
Components
BSA11M2
BioReady Hot Start Taq.
250units
10×Reaction Buffer (with 15 mM MgCl2 )
1000ul
2.5 mM dNTP Mixture
800ul
6×loading dye
500ul
ddH2O
1250ul
Features
High-Specificity
High activity
The PCR product with this enzyme is available to run TA clone directly.
The product is suitable for realtime PCR reaction owing to 5’-3’ exonuclease activity.
Application
PCR reaction
TA clone
Sequence
Experimental Data
M1: DL 2000
Lane 1, 3: BioReady Taq
Lane 2, 4: BioReady Hot Start Taq
M2: DL 15000
