BioReady Pfu is a thermostable enzyme of approximately 92 KD isolated from pyrococcus furiosus. BioReady Pfu catalyzes the DNA-dependent polymerization of nucleotide into duplex DNA in the 5’-3’ direction in the presence of magnesium ions. The enzyme also exhibits 3’-5’ exonuclease (proofreading) activity. The base misinsertions that may occur infrequently during polymerization are rapidly emendated by the proofreading activity of the polymerase. In generally, BioReady Pfu is mainly used for polymerization reactions requiring high fidelity synthesis.
Components
BSA10M2
BioReady Pfu Taq.
250units
10×Reaction Buffer (with 15 mM MgCl2 )
1000ul
2.5 mM dNTP Mixture
500ul
6×loading dye
500ul
ddH2O
1250ul
Features
High fidelity
Good thermostable
High activity
5’-3’ exonuclease activity
Application
PCR reaction
Sequence
Experimental Data
1. Electrophoresis result
2. Sequence result
M: DL 2000
Lane: GAPDH 400bp
