rt007 amv reverse transcriptase was isolated from avian myeloblastosis virus (amv) as the holoenzyme of molecular weight 157,000 daltons, using a modification of the method described by houts et al. this preparation is free of nuclease. it is qualified for cdna synthesis and also for dideoxy sequencing of dna and rna. total rna or poly a+ rna can be used as template, and optimal temperature is 42℃~-55℃, up to 60 ℃. if nappi is used, 37-41℃ is preferred. rt reaction buffer covered by patents can be used either as 5x /10x stock depending on different purpose (details in standard application). up to 10-12kb cdna may be obtained according to standard application.

comparison between amv reverse transcriptase and mmlv reverse transcriptase

	rt 007 amv	general m-mlv	rnaseh-m-mlv
reaction temperature	42-60℃	37-42℃	42-50℃
product length	10-12kb	3-5kb	6-8kb
origin	amv, holoenzyme	mmlv	clone expression
reaction efficieny	5u/reaction	200u/reaction	200u/reaction
rnase h activity	low and inhibited	strong	low
sensitivity	high	low	high
price	low	low	high

features:
high-sensitivity
high-yield
patented rt reaction buffer is suitable for many kinds of applications
up to 10-12kb cdna
up to 60 ℃ reaction temperature effectively opens the secondary structure of rna.

experimental data

③1μg hela total rna was reverse transcripted by rt enzymes from different companies(according to each protocol), then to run pcr. this is the electrophoresis result.

④1μg hela total rna was reverse transcripted by rt enzymes from different companies(according to each protocol), then to run ｒｅａｌ－ｔｉｍｅ　pcr（ｓｙｂｒ　ｇｒｅｅｎⅰ）.　this is the result　ｏｆ　ｃｔ　ｖａｌｕｅ.

order information