Lymphocytes Separation Medium uses a density grads principle to isolate lymphocytes (including monocytes). The media is used to isolate lymphocytes and monocytes from human peripheral blood. In general, with the buffer, you can get 70% or more lymphocytes and monocytes from the sample.
Features:
The isolated lymphocytes have high purity;
The operation is convenient and time-saving;
Using high quality Meglumine Diatri-zoate to make Lymphocytes Separation Medium, the separated monocytes has a high purity and yield.
Procedure
Add the Lymphocytes Separation Medium
Add anticoagulant and Hank mixture
Centrifuge
The liquid will be dibided into several phases
Absorb the mononuclear cells
wash
Discard the supernatant and repeat to wash again
Application
Lymphocytes Separation Media can be used to isolate lymphocytes and
monocytes from 1ml~ 5 ml human peripheral bloods. Yielded intact lymphocytes and
monocytes for further use.
This media is not intended to deal with the whole bloods in animal’s species.