Due to the corrosive nature of the phenol, preparation of phenol solution for use in a molecular biology laboratory is a time-consuming and ofen hazardous procedure. Frequently it is necessary to redistill the phenol prior to use to remove contaminants and oxidation products that can damage nucleic acids. In some cases, the PH phenol solutions needs to be adjusted before use. Safety precautions such as protective eyewear, gloves, and a fume hood are necessary when you use the product.
Features
We have a full line of products such as equilibrated phenol, mixed phenol, saturated phenol and so on.
Manufactured under the standard of ISO9001 and strict quality control
It can be applied for RNA and DNA extraction and there is no contamination of RNase, DNase enzyme
We provide convenient, safe and multiple types of phenol solutions to customers to avoid many complicated and dangers operations.
Procedure
Efficient extraction of cell extracts or solutions containing nucleic acids are most often performed with a series of phenol and phenol-chloroform extractions at a specific pH. Both phenol and chloroform cause proteins to become denatured and become soluble in the organic phase or interphase, while nucleic acids remain in the aqueous phase. After centrifugation, the aqueous phase containing nucleic acids is re-extracted with an equal volume of chloroform-isoamly alcohol. This combination of extractions is to reduce the loss of RNA due to the formation of insoluble protein: RNA complexes at the interphase.
Chloroform is mixed with phenol to increase the efficiency of nucleic acid extractions by reducing losses of RNA at the interphase. The increased efficiency is due to chloroform’s ability to denature proteins and aid in the removal of lipids, thus improving separation of nucleic acid into the aqueous phase. Phase separation is also enhanced, which assists in the removal of the aqueous phase with minimal cross contamination from the organic phase. In general, isoamyl alcohol is added to phenol-chloroform to reduce foaming.
Product |
Application |
Remarks |
Equilibrated Phenol
Equilibrated with Tris-HCl, pH8.0, 1.0 mM EDTA Phenol phase pH 7.8±0.2 |
Nucleic acid extraction
(DNA/RNA) |
Add oncluded buffer to increase pH to 8.0 |
Saturated Phenol
Saturated with citrate buffer
pH 4.3±0.2 |
Total RNA extraction, can remain total RNA alone in the aqueous phase |
At an acidic pH, below pH 7.0, DNA will be denatured and partition into the organic phase. And interphase, leaving the RNA alone in the aqueous phase. |
Phenol:Chloroform:IAA, pH 7.9
Equilibrated with Tris-HCl, pH 8.0, 1.0mM EDTA Phenol phase pH 7.8±0.2 |
Nucleic acid extraction
(DNA/RNA) |
Chloroform and IAA help stabilize interface and prevent foaming when mixing |
Water Saturated Phenol |
Total RNA extraction, can leaving total RNA alone in the aqueous phase |
It has an acid pH value, which allows direct use for RNA extraction |
Order Information
| Cat# |
Name |
Specification |
Remarks |
BSA02M1 |
Equilibrated Phenol
Equilibrated with Tris-HCl, pH8.0, 1.0 mM EDTA Phenol phase pH 7.8±0.2 |
100ml |
Stored at 4℃ |
BSA03M1 |
Phenol:Chloroform:IAA, pH 7.9
Equilibrated with Tris-HCl, pH 8.0, 1.0mM EDTA Phenol phase pH 7.8±0.2 |
100ml |
Stored at 4℃ |
BSA04M1 |
Saturated Phenol
Saturated with citrate buffer
pH 4.3±0.2 |
100ml |
Stored at 4℃ |
BSA05M1 |
Water Saturated Phenol |
100ml |
Stored at 4℃ |
|
