Hepatitis B virus (HBV) is a member of the genus Orthohepadnavirus and the causative agent of hepatitis B. HBV is characterized by high replication efficiency and a high mutation rate. At least eight genotypes (A–H) have been identified, each with multiple subtypes, and recombination can occur between genotypes. In China, genotypes B, C, and D are the most prevalent.
Quantitative measurement of HBV DNA allows the assessment of viral load and replication activity in research samples. As a highly sensitive method, Nucleic Acid Amplification Testing (NAT) can detect low levels of HBV, monitor viral concentration, replication activity, infectivity, and evaluate the effects of experimental antiviral treatments. It is also a key tool for studying occult HBV and the dynamics of chronic HBV infection in research settings.
1.Sample Type: Serum, Plasma
2.High Accuracy: Effectively quantitatively the content of hepatitis B virus in the sample, and the result meets expectations.
3.Good Specificity: No cross reactivity has been observed by testing the positive specimens such as Human cytomegalovirus, Influenza A virus, EB virus, Human immunodeficiency virus, Hepatitis C virus, Herpes simplex virus I, Herpes simplex virus II, Staphylococcus aureus and Canidia Albicans.
4.Real-time Monitoring: The introduction of external and internal control is used to monitor the entire extraction and PCR detection process.
5.Simple Operation: The fully premixed reaction solution can be directly dispensed when used, and the tube is completely closed to complete the amplification and detection process, effectively preventing aerosol contamination.
Disease Progression Monitoring
Prevention of Mother-to-Child Transmission
Blood Safety
Epidemiological Surveillance
Drug Resistance Testing
The low-concentration hepatitis B virus nucleic acid sample was tested repeatedly with this kit. The coefficient of variation of the Ct value was less than 2%, and the coefficient of variation of the logarithmic value of the quantitative concentration was less than 5%, indicating that the kit has good reproducibility. The test results are stable and reliable, and the test results are as follows:
Using the kit to detect hepatitis B virus and draw a standard curve, the correlation coefficient of the Ct value of the target gene in the unknown sample is above 0.995, indicating that the kit has a good linear relationship and high PCR efficiency. The result is shown in the figure as below:
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25°C--15°C away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit inthe period of validity).
| Cat# | Product Name | Packing size |
| BSB01M1 | HBV PCR Fluorescence Quantitative Detection Kit | 48 T |
| BSB01L1 | HBV PCR Fluorescence Quantitative Detection Kit | 96 T |