The kit was used for qualitative detection of mycoplasma in treated human cell culture fluids, including cell banks, virus seed, and clinical therapeutic cells and biological products. The test is indicated for use as an aid in diagnosis of the mycoplasma infection and should not be used as the sole basis for clinical diagnosis and treatment or management of patients.
PRINCIPLE
This kit was designed to detect the relatively conserved region of mycoplasma genome respectively by specific primers and fluorescent probes and performs rapid qualitative detection of the pathogens DNA by qPCR after the nucleic acid extraction process. In the extraction and detection process, Exogenous plasmid was introduced as a non-competitive internal control to monitor the whole extraction and detection process. In addition, UDG enzyme anti-contamination system was added in the kit to fully decompose possible product contamination and avoid false positive results. The kit mixes all the components in advance, and the experiment can be carried out without additional preparation actions, which is simple and convenient to use.
