Product Details FAQ Specification Instructions

This kit is designed for one-step reverse transcription (RT) PCR amplification in a single tube. The formulation allows us just adding primers, probes and templates. Therefore, the risk of contamination during operations is minimized. The optimized reverse transcriptase, RNase inhibitor and hot-start Taq DNA polymerase together with a one-step reaction buffer result in high amplification efficiency, high detection sensitivity and less non-specific amplification.

This kit includes all reagents necessary for RT from RNA to cDNA and second strand cDNA synthesized. Furthermore, electrophoresis loading dyes and a high-density reagent are included in the reaction mixture to allow visual confirmation of gel loading and facilitate gel loading at electrophoresis after PCR. Consequently, the PCR products with a single A nucleotide in 3’ end can be used for TA cloning.

1. High specificity: The antibody-modified hot-start Taq polymerase effectively suppresses nonspecific PCR amplification, enhancing the sensitivity and specificity of the reaction.

2. High Sensitivity: 0.1pg RNA.

3. Simple and convenient: Before the reaction, you only need to add primers and templates, reducing the risk of contamination from multiple pipetting steps. After the reaction, you can directly proceed to electrophoresis.

• Polymerase Chain Reaction (PCR)
• Site-Directed Mutagenesis
• Cloning
• Sequencing
• Genotyping
• Gene Expression Analysis

1. The experiment involved comparing the amplification bands obtained from QH9 samples using BIOER, T brand, and V brand reagents. The results demonstrated that all the samples were effectively amplified. Moreover, using the BioRT Master One Step RT-PCR Kit III (with dye) produced a single and brighter amplification band compared to similar products from V and T companies.

2. The experiment, which included RT-PCR product gel electrophoresis, assessed the performance and stability of the BioRT Master One Step RT-PCR Kit III (with dye). The results demonstrated that BSB72 can effectively amplify total RNA as low as 0.1 pg, resulting in a single amplification band with a product length of 1090 bp.