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Monkeypox virus is a kind of enveloped double stranded DNA virus, which is rectangular in shape and can be cultured and grown in African green monkey kidney cells, causing cytopathic effects. It belongs to the orthopoxviral genus of the poxviridae family. Monkeypox is a zoonotic infectious disease caused by monkeypox virus primers. Its clinical manifestations include high fever, headache, general discomfort, cough, enlarged lymphnodes, accompanied by a rash similar to smallpox throughout the body, and occasional abdominal pain. This kit uses fluorescence PCR method and can be applied to Bioer’s LineGene 9600 Plus (FQD-96A), Bioer’s QuantGene 9600 (FQD-96C) and ABI 7500. It has the characteristics of fast detection speed, good specificity, and high sensitivity.


Product Details FAQ Specification Instructions

This kit selects two highly conserved regions of monkeypox virus, the F3L gene (FAM) and the B6R gene (HEX) and designs two pairs of specific primer probes. The primers and probes can specifically bind to the target sequence. Under the action of Taq enzyme, the fragment is specifically amplified, and the fluorescent probe is hydrolyzed during the amplification process to produce fluorescence. The detection system contains dUTP-UDG enzyme anti-pollution measures to fully degrade possible product contamination and avoid false positive results. In addition, internal standards are set up to monitor the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification, avoiding false negative results, and ensuring the effectiveness of the entire process.


Advantages

This kit can qualitatively detect monkeypox virus nucleic acid, providing information for clinical diagnosis; The detection system contains dUTP-UDG enzyme anti-pollution measures to fully degrade possible product contamination and avoid false positive results; At the same time, introduce human derived internal reference materials to monitor the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification to avoid false negative results. It takes a short time and can complete the detection report within 30 minutes.

� Strong applicability

Suitable for pathological swab samples.

� High sensitivity:

Three different batches of reagents are used for testing, and the detection sensitivity can reach 150 copies/mL.

� Strong specificity

No cross reaction with various common pathogens such as enterovirus 71, measles virus, rubella virus, varicella zoster virus, herpes simplex virus I, herpes simplex virus II, Epstein Barr virus, cytomegalovirus, human herpesvirus 6, and human herpesvirus 7.

� High accuracy

It can quickly detect monkeypox virus. Test results of reference materials: positive coincidence rate 100%, negative coincidence rate 100%.

� Simple operation
Fully closed tube amplification and detection to prevent aerosol pollution.


Monkeypox Virus Nucleic Acid Detection Kit (Fluorescent PCR) is used for the qualitative detection of Monkeypox Virus nucleic acid in lesion swab specimens from suspected cases.





1. The kit should be stored at -25°C ~ -15°C away from light and avoid repeated freeze-thaw.

2. The kit can be stored for 7 days at 2-8°C after opening.

3. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.

4. The kit can be transported in foam box sealed with ice bags or dry ice under 8°C.


Cat# Product Name Packing size
BSJ34S1 Monkeypox Virus Nucleic Acid Multiple Detection Kit (Fluorescent PCR) 24T
BSJ34M1 Monkeypox Virus Nucleic Acid Multiple Detection Kit (Fluorescent PCR) 48T