This kit selects highly conserved regions of Streptococcus pneumoniae and Haemophilus influenzae, and designs specific primer probes. The primers and probes can specifically bind to the target sequence, and under the action of Taq enzyme, the fragment can be specifically amplified, and the fluorescent Probes are hydrolyzed during amplification, resulting in fluorescence. The detection system contains dUTP-UDG enzyme anti-pollution measures to fully degrade possible product contamination and avoid false positive results; in addition, an internal standard is set, which is a highly conserved region on the human genome, and the internal standard monitors sample collection, transportation, nucleic acid extraction and PCR amplification. Increase the whole process, avoid false negative results, and ensure the effectiveness of the whole process.
Advantages
This kit can qualitatively detect the nucleic acids of Streptococcus pneumoniae and Haemophilus influenzae, providing information for clinical diagnosis; the detection system contains dUTP-UDG enzyme anti-pollution measures, which can fully degrade possible product contamination and avoid false positive results; At the same time, human-derived internal references are introduced to monitor the entire process of specimen collection, transportation, nucleic acid extraction, and PCR amplification to avoid false negative results; it takes only 32 minutes to complete detection and issue report.
Streptococcus Pneumoniae and Haemophilus Influenzae Nucleic Acid DetectionKit (Fluorescence PCR) is used for the qualitative detection of the StreptococcusPneumoniae (SP) and Haemophilus Influenzae (Hl) nucleic acid in specimens ofsputum from suspected cases. The kit is used for the auxiliary diagnosis andepidemiological surveillance of SP and HI infection, cannot be used as the basis forthe diagnosis or exclusion of cases alone.
For professional use only.
For in vitro diagnostic use only.
1. Accuracy: after the positive reference products P1-P10 and negative reference products N1-N10 of Hangzhou Bioer Technolo gy Co., Ltd. are reconstituted as required, they are extracted with Bioer extraction kit BSC71S1E and tested.
3. Clinical specimen experiment: Bioer extraction kit BSC71S1E is used to extract and detect clinical specimens of Streptococcus pneumoniae and Haemophilus influenzae. At the same time, the detection kit of the same kind that have obtained NMPA certificate are used as a comparison to verify the compliance rate.